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KMID : 1145120200180030413
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Asian Journal of Beauty and Cosmetology
2020 Volume.18 No. 3 p.413 ~ p.422
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Skin Health Improving Effects of Korean Freeze-dried Royal Jelly in Human Keratinocytes
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Kim Hyo-Young
Choi Hong-Min
Kim Se-Gun
Woo Soon-Ok
Moon Hyo-Jung
Han Sang-Mi
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Abstract
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Purpose : In this study, we aimed to examine the anti-skin aging effects of Korean freeze-dried royal jelly and its main ingredient, 10-Hydroxydecanoic acid (10-HDA), in order to promote Korean royal jelly as a health functional food ingredient for improving skin health.
Methods : The effects of Korean freeze-dried royal jelly and 10-HDA were determined by examining the proliferation and migration of human keratinocytes (HaCaT cells) in a skin wound healing model by using EZ-cytox kit assay. The changes in production of matrix metalloproteinase (MMP)-1 and MMP-3, which are related to skin aging in HaCaT cells, after UV-B irradiation were analyzed using enzyme-linked immunosorbent assay. Real-time polymerase chain reaction was employed to analyze MMP-1 and MMP-3 gene expression.
Results : Korean freeze-dried royal jelly and 10-HDA improved the regeneration and migration of mechanically damaged HaCaT cells. The test group treated with 100 μg/mL of Korean freeze-dried royal jelly and 100 μM of 10-HDA yielded similar results to those obtained for the positive control group treated with vitamin C. Furthermore, Korean freeze-dried royal jelly and 10-HDA significantly reduced the production and gene expression of MMP-1 and MMP-3, which are the key elements that induce skin aging by suppressing collagen generation in UVB-irradiated HaCaT cells.
Conclusion : We examined the anti-wrinkle effects of Korean freeze-dried royal jelly and its main ingredient, 10-HDA. The results suggested the applicability of Korean royal jelly as a key functional health food ingredient for improving skin health.
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KEYWORD
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Royal jelly, 10-Hydroxydecanoic acid, Health functional food, Human keratinocyte, Anti-wrinkle
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